Fig 1: STRING protein-protein interaction networks of immunosuppressive DEGs. STRING protein-protein interaction networks of upregulated (red) and downregulated (blue) DEGs directly associated with a CD24, b THBS1 and THBS2, and c CD276. The list of transcripts used to create these networks was derived from all DEGs associated with clusters 1, 2, and 4. Each node indicates a specific protein, shaded according to its log2 fold change value (PEG vs. NC comparison). Pink = upregulated, blue = downregulated. Distances between nodes indicates greater or lesser association between proteins, while the thickness of the lines between nodes indicates the confidence score of the interaction (thicker = greater confidence, thinner = lesser confidence). The positions of CD24, THBS1, THBS2, and CD276 in each network are indicated by red borders
Fig 2: Immunohistochemical analyses of cell adhesion and extracellular matrix DEGs. a Fluorescence images showing cross-sections of unoperated control nerve, 14 days PO PEG-fused PNA, and 14 days PO NC PNA immunostained for COL1A1 (green). b Percent (%) COL1A1 staining area comparisons at 14 days PO (n = 4 per treatment group). c Normalized read counts of COL1A1 RNA transcripts at 14 days PO. Fluorescence images showing immunostaining for CD24 (red) in cross-sections. d Fluorescence images showing immunostaining for CD24 (red). e Percent (%) CD24 staining area comparisons at 14 days PO (n = 4 per treatment group). f Normalized read counts of CD24 RNA transcripts at 14 days PO. Fluorescence images showing immunostaining for CD24 (red) in cross-sections. g Fluorescence images showing immunostaining for THBS1 (red). h Percent (%) THBS1 staining area comparisons at 14 days PO (n = 4 per treatment group). i Normalized read counts of THBS1 RNA transcripts at 14 days PO. j Fluorescence images showing immunostaining for THBS2 (red). k Percent (%) THBS2 staining area comparisons at 14 days PO (n = 4 per treatment group). l Normalized read counts of THBS2 RNA transcripts at 14 days PO. m Fluorescence images showing immunostaining for CD276 (red). n Percent (%) CD276 staining area comparisons at 14 days PO (n = 4 per treatment group). o Normalized read counts of CD276 RNA transcripts at 14 days PO. Nuclei are stained with DAPI (blue). Scale = 50 µm. In graphs, data are shown as mean (horizontal bar) with each individual data point per animal. In b, e, h, k, and n, overall one-way ANOVA p values are shown above brackets at each time point, with multiple comparisons p values between treatment groups displayed underneath
Supplier Page from Thermo Fisher Scientific for Thrombospondin 2 Antibody